Abstract: Vickers hardness is a standard to express the hardness of materials, and the evaluation method of the uncertainty of vickers hardness measurement can make the measurement of vickers hardness more accurate and reliable.This article respectively using FW214 types of vickers hardness tester by comprehensive evaluation method, for by the state professional measuring unit calibration 459HV10 standard blocks were tested respectively, and the measurement results of influence factors were analyzed, and evaluation of uncertainty of measurement of the vickers hardness test.The results showed that the testing level could meet the error range of GB/T 4340.2. The comprehensive evaluation method can not only improve the test efficiency but also meet the requirement of experimental accuracy.