Abstract: In order to shorten the development cycle of new materials and reduce the R&D cost, a high-throughput hardness tester with 32 stations was developed by designing the related machine control system and software based on the characteristics and mechanism of hardness test, thus realizing the high-throughput characterization of mechanical properties of materials. The automatic dotting, automatic recording and automatic switching of samples could be realized in hardness testing. The testing time of the developed high-throughput hardness tester was only 1/3 of the common manual hardness tester, which significantly enhanced the characterization rate of material property. The developed high-throughput hardness tester was applied for the testing of standard samples with different hardness, stainless samples, and high-entropy alloy samples. The results showed that the high-throughput tester hardness was suited for the material with hardness greater than 200HV (∽19 HRC). The indication error and indication repeatability were -0.2HRC and 0.764HRC, respectively. The measuring accuracy could meet the requirements of hardness tester, and the measuring results were reliable.