Determination of Curie temperature of amorphous alloy Fe78Si9B13 by differential scanning calorimetry was researched from the choices of crucibles, masses of the sample and heating rates, and then the optical test conditions were obtained. The relationship of Curie temperature of amorphous alloy Fe78Si9B13 with heating rates was analyzed and compared simultaneously with the relationships of crystallization temperatures of Fe78Si9B13, melting temperature of In (standard material used for thermal analysis) with the heating rates. The results show that the heat signal of the Curie transition shows a little wave in the DSC curves. The Curie temperature obtained at different test conditions are about from 399℃ to 402℃, which are equivalent to the results obtained by the methods according to Hall effect. The best conditions are as follows: Al crucibles, mass of about 6mg, heating rate of 20K/min. It is found that the Curie signal can be obtained only as heating rates bigger than 10K/min and the results are not affected obviously by the heating rates when they are increased because of the low heat as no thermal hysteresis affect the results, while the results of crystallization temperatures increase obviously for the thermal hysteresis.