Abstract: 【Objective】 To prepare spherical Mo-Re alloy powder with high purity, high sphericity, and excellent physical properties for additive manufacturing, and to characterize and evaluate its performance, thereby providing a reference for the application of Mo-Re alloy in the field of additive manufacturing, spherical Mo-Re alloy powder was prepared by the Plasma Rotating Electrode Process（PREP） using electrodes fabricated by electron beam melting and arc melting. 【Method】 The microstructure and physical properties of the spherical Mo-Re alloy powder were investigated through chemical analysis, metallographic examination, particle size analysis, sphericity measurement, and physical property testing. 【Result】 The prepared spherical Mo-Re alloy powder exhibited oxygen and nitrogen contents below 0.005%, with the main alloying element Re meeting the target composition and being well solid-solutioned. The powder achieved a sphericity of 0.979, a D50 particle size of 117.59 μm, a tap density of 6.5 g/cm³, and a flowability of 8.2 s/50 g. 【Conclusion】 The Mo-Re alloy powder prepared by PREP demonstrates high purity and excellent performance. It features high sphericity, good surface quality, and superior physical properties, making it suitable for powder-bed-based 3D printing. However, due to its relatively coarse particle size and low yield of fine powder, it is more appropriate for electron beam melting processes that require coarser powder fractions.