Abstract: With the increasing demand for high safety and lightweight in the automotive industry, advanced high strength steels are widely used in key automotive components. However, the problem of hydrogen embrittlement failure during service has become increasingly prominent. Therefore, developing advanced automotive high strength steels that combine high strength and ductility with low hydrogen embrittlement sensitivity has become a critical challenge. Transformation induced plasticity （TRIP） steel contains metastable retained austenite, offering both excellent strength and ductility and potential hydrogen embrittlement resistance. In this study, a pre-quenching heat treatment process was introduced to systematically investigate the effect of microstructure evolution on the hydrogen embrittlement behavior of TRIP steel. The results show that the pre-quenching treatment significantly changes the microstructure morphology of the steel. The matrix transforms from an equiaxed structure to a structure with a layered feature, and the formation and stabilization of retained austenite are promoted. Compared with conventional TRIP steel, the pre-quenched TRIP steel exhibits a significantly higher volume fraction of retained austenite, which is predominantly film-like in morphology and shows higher stability. In addition, the pre-quenching treatment refines the grain size and increases the proportion of special grain boundaries such as Σ3, which helps to inhibit hydrogen segregation at grain boundaries and intergranular cracking. Meanwhile, the nano-scale precipitates in the pre-quenched TRIP steel show a finer and more dispersed distribution. Slow strain rate tensile test （SSRT） and thermal desorption spectroscopy （TDS） analysis results demonstrate that the pre-quenched TRIP steel exhibits lower hydrogen embrittlement sensitivity under different hydrogen charging conditions. The formation of multi-scale high binding energy hydrogen trap structures in its microstructure effectively inhibits the involvement of diffusible hydrogen in hydrogen-induced damage. Therefore, the pre-quenching treatment establishes a multi-scale hydrogen trapping system through the synergistic effects of stabilizing retained austenite, refining precipitates, and optimizing grain boundary structures, achieving a synergistic optimization of strength, ductility, and hydrogen embrittlement resistance.