Abstract: Alloying is an important way to improve the comprehensive properties of zinc-aluminum alloy. The accurate and rapid determination of alloying elements(including bismuth, chromium, manganese, nickel and titanium) is crucial to the product quality control and the development of high-performance alloy. In this study, the sample was dissolved with dilute aqua regia. Bi 223.061 nm, Cr 267.716 nm, Mn 257.611 nm, Ni 231.604 nm and Ti 336.121 nm were selected as the analytical lines. High-purity zinc was used for matrix matching to eliminate the matrix effect. The method for the simultaneous determination of five alloying elements(bismuth, chromium, manganese, nickel and titanium) in zinc-aluminum alloy by inductively coupled plasma atomic emission spectrometry(ICP-AES) was established. The linear correlation coefficients of calibration curves for testing elements were all higher than 0.999 5. The limits of detection of this method were between 0.02 and 0.43 μg/g. The contents of bismuth, chromium, manganese, nickel and titanium in three certified reference materials of zinc-aluminum alloy and two zinc-aluminum alloy actual samples were determined according to the experimental method. The measurement results of certified reference materials were consistent with the certified values. For the actual samples, the relative standard deviations(RSD, n=8) of determination results ranged from 0.48% to 1.9%, and the spiked recoveries were between 94% and 106%.