Abstract: In order to investigate the influence of hot rolling reduction on the microstructural evolution throughout the entire processing route and the final mechanical and magnetic properties of non-oriented electrical steel produced by twin-roll strip-casting technology, a twin-roll strip-casting non-oriented electrical steel with a designed composition of Fe-3.35Si-1.37Mn(mass fraction, %)was used as the research object. Single-pass hot rolling tests with different reductions were carried out. Subsequently, all samples were uniformly cold-rolled to 0.27 mm to ensure the same total rolling reduction and then subjected to decarburization annealing to complete the preparation of finished products. The mechanical and magnetic properties of the finished products were tested. The results show columnar grains inclined and fractured in the central region during hot rolling. With the increase of hot rolling reduction, the fraction of columnar grains decreases gradually until they are completely transformed into equiaxed grains. A further increase in hot rolling reduction leads to a gradual increase in the amount of fibrous microstructures and a continuous rise in the fraction of 111 oriented texture which exerts a deteriorating effect on the subsequent magnetic properties. With the increase of hot rolling reduction, the cold rolling reduction decreases correspondingly, the cold rolling deformation stored energy decreases significantly, the driving force for annealing recrystallization is weakened and abnormal grain growth is caused. Under the condition of the same total rolling reduction, the higher the annealing temperature, the smaller the influence of stored energy differences of grains with different orientations on the growth process of recrystallized grains and the more uniform the grain size distribution. With the increase of hot rolling reduction, the magnetic induction (B₅₀) shows a gradually decreasing trend, the low frequency core losses(P_1.5/50) and medium frequency core losses(P_1.0/400) increase gradually, and the yield strength and tensile strength show a trend of first increasing and then decreasing. Under the laboratory conditions of this study, the non-oriented electrical steel with both excellent magnetic properties and good mechanical properties can be prepared when the hot rolling reduction is 25.0%-37.5%, the cold rolling reduction is 80% and the annealing system is 1 100 ℃ for 2 min. This study clarifies the influence law of hot rolling reduction on the full-process microstructure inheritance and property regulation of cast-rolled silicon steel in the "hot rolling-cold rolling-annealing" process under the same total rolling reduction and provides a theoretical basis and process window for the production of sub-0.30 mm high-grade non-oriented electrical steel by twin-roll strip-casting technology.